Regulatory

Part:BBa_K3030015

Designed by: Xiaomeng Liu   Group: iGEM19_XJTLU-CHINA   (2019-09-13)


theophylline riboswitch with optimized RBS

This is a theophylline-responsive riboswitch containing optimized RBS. The sequence is initially designed by Wieland, M. and Hartig, J. S. (2008) with Shines-Dalgarno (SD) sequence. We optimized the RBS by replacing SD sequence with calculated RBS by thermodynamic modeling. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

Wieland, M., Hartig, J.S. (2008). ‘Improved aptazyme design and in vivo screening enable riboswitching in bacteria.’ Angewandte Chemie, 47(14), pp2604-2607. [Online]. Available from: https://www.ncbi.nlm.nih.gov/pubmed/18270990


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